Analyze data
Follow this procedure to process your single molecule videos (SMVs) or trajectories and characterize the molecule dynamics in your sample.
Note: Skip step 1 if already in possession of intensity-time traces (ASCII files or in a mash project).
- Step 1: Create traces
- Step 2: Find states in traces
- Step 3: Identify state network
- Step 4: Determine state populations
<< PREVIOUS: Find states in traces NEXT: Determine state populations >>
Step 3: Identify state network
In this step, the sample’s most sufficient state configuration is identified and state transition rates are estimated from transitions detected in single molecule traces.
Note: Alternatively, if states are sufficiently separated, the most sufficient state configuration can be identified from histogram data; see Workflow for more information.
In construction
<< PREVIOUS: Find states in traces NEXT: Determine state populations >>