Dwell time statistics

Dwell time statistics files are ASCII files with the extension .kin. They are usually found in the main/traces_processing/dwell-times analysis folder.

Table of contents

  1. Description
  2. File name
  3. Structure

Description

Dwell time statistics files are written in ASCII format and contain statistics on state transitions and state durations in all exported state trajectories.

They are created in the trace_processing/dwell-times analysis sub-folder after exporting .kin files from window Export options of module Trace processing.

File name

The file is named by the user during the export process.

By default the file is named after the selected project file and is appended with the extension _[Ddd], where [Ddd] the data type written in the file.

Data types supported in dwell time statistics files are:

  • I[i]-[L]: intensities in detection channel indexed [i] upon illumination with laser wavelength [L] nm
  • FRET[D]to[A]: FRET from donor emitter detected in channel indexed [D] to acceptor emitter detected in channel indexed [A]
  • S[D]to[A]: stoichiometry associated to donor emitter detected in channel indexed [D] and acceptor emitter detected in channel indexed [A]

Structure

Dwell time statistics are organized column-wise with:

  • column file name containing names of exported dwell time files from which statistics are calculated
  • columns state1 and state2 containing state values before and after transition respectively
  • columns total time spent in [j] containing summed durations of state [j] (in second)
  • columns average time in [j] containing average duration of state [j] in the trajectory (in second)
  • columns ratio average time 1/2 containing the ratio of the average dwell time before transition over the average dwell time after transition; this can be used as an approximation of the thermodynamic constant for a two-state system
file name	state1	state2	total time spent in 1	total time spent in 2	average time in 1	average time in 2	ratio average time 1/2
sim_mol1of85_FRET1to2.dt	2.068498e-02	1.000513e+00	18	17	1.637000e+02	2.321000e+02	9.094444e+00	1.365294e+01	6.661161e-01
sim_mol1of85_FRET1to2.dt	1.000513e+00	2.068498e-02	17	18	2.321000e+02	1.637000e+02	1.365294e+01	9.094444e+00	1.501240e+00
sim_mol2of85_FRET1to2.dt	2.265396e-02	1.000780e+00	19	19	1.343000e+02	2.503000e+02	7.068421e+00	1.317368e+01	5.365561e-01

If a state transition is not found reversible in the dwell time file, columns total time spent in [j] are set to 0 and columns average time in [j] as well as ratio average time 1/2 are set to NaN.