Control area

The control area consists in a navigation dashbord and the three main control buttons.

Use this area to browse molecules in the sample and to refresh or export single molecule data.

Area components

Defines the current molecule index.

The index of the molecule which is currently in display can be edited in (a) and the total size of the molecule sample is shown in (b).

Press or to display the previous or next molecule in the sample, respectively.

After changing the current molecule index, the Visualization area is updated and the processing parameters of panels Sub-images, Background correction, Factor corrections, Photobleaching, Denoising and Find states are adapted to the current molecule.

Press UPDATE to update corrections and calculations for the current molecule.

Operations include all intensity corrections as configured in panels Background correction, Re-sampling, Cross talks, Denoising, Photobleaching and Factor corrections, as well as calculations of state trajectories as configured in panel Find states.

Usually, this functionality is used after changing any processing parameters in the sub-mentioned panels.

After processing, input data in modules Histogram analysis and Transition analysis are immediately refreshed.

Press UPDATE ALL to update corrections and calculations for all molecules in the sample.

After processing, input data in modules Histogram analysis and Transition analysis are immediately refreshed.

Usually, this functionality is used before proceeding with histogram or transition analysis, saving the MASH project or opening the Trace manager.

Press EXPORT... to open the export options.

Export options defines the file formats to export, including ASCII files and figures.

To set export options, refer to Set export options.