Gamma factor files
Gamma factor files are ASCII files with the extension .gam
. They are usually found in the main/traces_processing/parameters
analysis folder.
Table of contents
The gamma factor files are written in an ASCII format and contains gamma factors to correct all FRET-time traces in the project.
They are created in the /trace_processing/parameters
analysis sub-folder after exporting .gam files from panel
Sample managementof module Trace processing.
File name
The file is named by the user during the export process.
By default, the file is named after the project file selected in Trace processing.
Structure
FRET pair-specific gamma factors are organized column-wise with each column corresponding to a FRET pair, and row-wise by appending the same columns for each molecule.
FRET1-2 FRET1-3 FRET2-3
1.000 1.000 1.000
0.825 1.000 0.900
0.900 1.000 1.000
FRET pairs are organized as in the list FRET calculations.
Compatibility
Gamma factor file can be imported:
- along with ASCII traces in MASH’s module Trace processing by adjusting the Import options
- after import from panel Factor corrections