Transformation files

Transformation files are binary Matlab files with the extension .mat. They are usually found in the main/video_processing/coordinates/transformed analysis folder.

Table of contents

  1. Description
  2. File name
  3. Structure
  4. Compatibility

Description

The transformation file is written in the MATLAB binary format and contains the channel transformation.

It is created in the /video_processing/coordinates/transformed analysis sub-folder when calculating and exporting the channel transformation in panel Coordinates transformation of module Video processing.

File name

The file is named by the user during the export process.

By default, the file is named after the reference coordinates file loaded in panel Coordinates transformation.

Structure

Transformations are created from reference coordinates with the MATLAB build-in functions:

  • fitgeotrans for MATLAB versions newer than 9.0 (R2016a)
  • cp2tform otherwise

Please visit the respective sections of MATLAB’s documentation (cp2tform and fitgeotrans) for more information about the file content.

Compatibility

Transformation files can be imported as channel transformation in panel Coordinates transformation to transform spots coordinates to single molecule coordinates.

Transformation files are MATLAB binary files and can be imported in MATLAB’s workspace by simply drag-and-dropping the file, or by typing in MATLAB’s command window:

load('datafolder\transformation-file.mat','-mat');

and replacing datafolder\transformation-file.mat by your actual file name and directory.