Dwell time histogram files

Dwell time histogram files are ASCII files with the extension .hdt. They are usually found in the main/transition_analysis/kinetics folder.

Table of contents

  1. Description
  2. File name
  3. Structure

Description

The dwell time histogram files are written in ASCII format and contain state dwell time distributions prior a specific state transition.

They are created in the /transition_analysis/kinetics analysis sub-folder when exporting dwell time histograms from widow Export options of module Transition analysis.

File name

The file is named by the user during the export process.

By default, the file is named after the selected project file and is appended with the extension _[Ddd]_[j]to[j'], where [Ddd] is the data type written in the file and [j] and [j'] are the respective x- and y- coordinates of the transition cluster.

Data types supported in dwell time histogram files are:

  • I[i]-[L]: intensities in detection channel indexed [i] upon illumination with laser wavelength [L] nm
  • FRET[D]to[A]: FRET from donor emitter detected in channel indexed [D] to acceptor emitter detected in channel indexed [A]
  • S[D]to[A]: stoichiometry associated to donor emitter detected in channel indexed [D] and acceptor emitter detected in channel indexed [A]

The extension _[Ttt] is added to the data type [Ddd] if a particular subgroup of molecules is analyzed, with [Ttt] the corresponding molecule tag.

Structure

Dwell time histograms are organized column-wise with:

  • column dwell-times(s) containing binned dwell times
  • column count absolute histogram count
  • column norm. count histogram count normalized by the sum
  • column cum. count absolute cumulative histogram count
  • column compl. norm. count complementarity of the cumulative histogram count normalized by the maximum
dwell-times(s)	count	norm. count	cum. count	compl. norm. count
0		0	0		0		1
1.017500e-01	17	2.575758e-01	17		7.424242e-01
2.035000e-01	8	1.212121e-01	25		6.212121e-01