Cross-talks correction
Cross-talks correction is the fifth panel of module Trace processing.
Access the panel content by pressing
.
The panel closes automatically after other panels open or after pressing
.
Cross-talks settings apply to the whole set of molecules.
Corrections will be applied only after processing data by pressing
; see
Process all molecules data for more information.
Use this panel to configure global bleedthrough and direct excitation coefficients used in Cross-talk correction.
Panel components
Emitter list
Use this list to select the emitter to configure or show the cross-talks for.
Corresponding bleedthrough and direct excitation coefficient are shown in Bleedthrough coefficients and Direct excitation correction, respectively.
Cross-talks are global correction coefficients, which means that only one set of coefficients is applied for the entire project.
Bleedthrough coefficients
Use this interface to define the bleedthrough coefficients.
The fluorescence of each emitter used in the experiment has the propensity to “bleed” through unspecific detection channels. The ratio of fluorescence signal detected in the unspecific channel over the signal detected in the specific channel is called the bleedthrough coefficient.
To set the bleedthrough coefficient of a particular emitter in a particular unspecific channel, select the emitter in the Emitter list, the destination channel in menu (a) and set the coefficient in (b).
For more information about how to obtain the bleedthrough coefficient and how correction is performed, please refer to Cross-talk correction in Trace processing workflow.
Direct excitation coefficients
Use this interface to define the direct excitation coefficients.
Each emitter used in the experiment has the propensity to be excited by unspecific lasers. The ratio of fluorescence signal detected upon unspecific illumination over the signal detected upon specific illumination is called the direct excitation coefficient.
To set the direct excitation coefficient of a particular emitter by a particular unspecific laser, select the emitter in the Emitter list, the unspecific laser in menu (a) and set the coefficient in (b).
For more information about how to obtain the direct excitation coefficient and how correction is performed, please refer to Cross-talk correction in Trace processing workflow.