Spotfinder coordinates files
Spotfinder coordinates files are ASCII files with the extension .spots
. They are usually found in the main/video_processing/coordinates/spotfinder
analysis folder.
Table of contents
Description
The spots coordinates file is written in ASCII format and contain pixel positions of bright spots detected with Spotfinder.
It is created in the /video_processing/coordinates/spotfinder
analysis sub-folder when saving spots coordinates in panel
Molecule coordinates of module Video processing.
File name
The file is named by the user during the export process.
By default, the file is named after the image/video file name loaded in Video processing.
Structure
Coordinates are written in double precision and are organized in a column-wise fashion with:
- columns
x
andy
containing x- and y- spots coordinates respectively - column
I
containing the pixel intensities at spots positions using Pixel intensity units - column
frame
containing frame indexes on which the spot detection was performed
x y I frame
1.450000e+01 3.450000e+01 518 1
7.350000e+01 2.150000e+01 542 1
1.350000e+01 1.405000e+02 547 1
In the case where the
Method settings include Gaussian fitting, the column I
contains Gaussian numeric integrals and the following columns and inserted:
assymetry
containing spot asymmetries in %, being equal of greater than 100%width
andheight
containing Gaussian standard deviations in the x- and y- direction respectively and given in pixeltheta
Gaussian orientation in radianz-offset
Gaussian offset given in Pixel intensity units
x y I assymetry width height theta z-offset frame
1.500000e+01 3.800000e+01 1.200463e+02 1.000000e+00 3.402311e-01 3.402311e-01 -3.230343e+04 1.129999e+03 1
1.033617e+02 1.942211e+02 1.212129e+02 1.162688e+00 8.045870e-01 6.920058e-01 -9.087937e+00 1.129771e+03 1
3.499995e+01 1.107173e+02 1.260142e+02 1.708943e+00 7.835743e-01 4.585141e-01 -1.169097e+05 1.129877e+03 1
Compatibility
Spots coordinates files can be imported for coordinates transformation in panel Coordinates transformation by adjusting the corresponding Import options to the actual file structure